Umami Taste Responses Are Mediated by -Transducin and -Gustducin
نویسندگان
چکیده
Wei He,1* Keiko Yasumatsu,3* Vijaya Varadarajan,1 Ayako Yamada,3 Janis Lem,4 Yuzo Ninomiya,3 Robert F. Margolskee,2 and Sami Damak1 1Department of Physiology and Biophysics and 2Howard Hughes Medical Institute, The Mount Sinai School of Medicine, New York, New York 10029, 3Section of Oral Neuroscience, Kyushu University, Fukuoka 812-8582, Japan, and 4Department of Ophthalmology and Program in Genetics, Tufts University School of Medicine and New England Medical Center, Boston, Massachusetts 02111
منابع مشابه
Partial Rescue of Taste Responses of α-Gustducin Null Mice by Transgenic Expression of α-Transducin
The transduction of responses to bitter and sweet compounds utilizes guanine nucleotide binding proteins (G proteins) and their coupled receptors. α-Gustducin, a transducin-like G protein α-subunit, and rod α-transducin are expressed in taste receptor cells. α-Gustducin knockout mice have profoundly diminished behavioral and electrophysiological responses to many bitter and sweet compounds, alt...
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The tastes of many bitter and sweet compounds are thought to be transduced via guanine nucleotide binding protein (G-protein)-coupled receptors, although the biochemical nature of these receptors is poorly understood at present. Gustducin, a taste-specific G-protein closely related to the transducins, is a key component in transducing the responses to compounds that humans equate with bitter an...
متن کاملFunctional interaction between TAS2R receptors and G-protein alpha subunits expressed in taste receptor cells.
Bitter taste perception is a conserved chemical sense against the ingestion of poisonous substances in mammals. It could be mediated by G protein-coupled receptors that need the appropriate G proteins to transduce the signals. TAS2R receptors and a G protein α subunit, α–gustducin are believed to be key molecules for its perception (Margolskee, 2002; Montmayeur and Matsunami, 2002), but little ...
متن کاملExpression of gustducin overlaps with that of type III IP3 receptor in taste buds of the rat soft palate.
Type III IP3 receptor (IP3R3) is one of the common critical calcium-signaling molecules for sweet, umami, and bitter signal transduction in taste cells, and the total IP3R3-expressing cell population represents all cells mediating these taste modalities in the taste buds. Although gustducin, a taste cell-specific G-protein, is also involved in sweet, umami, and bitter signal transduction, the e...
متن کاملFunctional expression of the taste specific G-protein, alpha-gustducin.
The taste-specific G-protein alpha-subunit, alpha-gustducin, was expressed using a baculovirus based system. alpha-Gustducin was demonstrated to be myristoylated and was also palmitoylated in insect larval cells. Recombinant alpha-gustducin was purified to homogeneity. Neither receptors nor effectors that interact with gustducin in taste are known. However, alpha-gustducin has a close structura...
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تاریخ انتشار 2004